New Approach for the Identification of Metabolites Using LC with Ion Mobility and Cryogenic Spectroscopy
Researchers from Polytechnique Fédérale de Lausanne in Switzerland have developed a new approach for identifying isomeric and isobaric metabolites using high-resolution ion mobility spectrometry (IMS) and cryogenic infrared (IR) spectroscopy. The complex structure of metabolites has made their identification challenging, and analytical standards are often required to confirm their presence in a sample. However, the new approach can separate isomeric metabolites in a matter of milliseconds and provide highly structured IR fingerprints for their unambiguous identification. Additionally, this approach allows for the automatic identification of metabolite isomers by comparing their IR fingerprints with those in a database, thereby eliminating the need for analytical standards.